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Courses in Practical DNA Microarray Analysis 2008

2008 May 5-8 in Munich

Registration is closed.

To get on the waiting-list, please send a short note that explains your existing skills and experience with (a) microarrays, (b) statistics, and (c) computer programming and your motivation for taking this course to t.beissbarth@dkfz.de.

If you bring your own laptop, be sure to have installed the following packages:
affy, Biobase, marray, multtest, limma, vsn, arrayMagic, cluster, RColorBrewer, hgu95av2, hgu95av2cdf, estrogen, ALL, annotate, genefilter, multtest, ROC, isis, pamr, e1071, MCRestimate, GlobalAncova, globaltest, GOstats, hgu133a, Rgraphviz.

Datasets and additional packages used in the practical sessions can be found in the data directory, links to the tutorial slides and practical exercises in the table below.

Preliminary Course schedule
 Monday, May 05 - First Analysis steps
09.00-09.30 Introduction and overview Ulrich Mansmann
09.30-12.30 Quality control, normalization and design Tim Beissbarth
14.00-17.00 Exercises: Introduction to R, cDNA data, affy data Mansmann, Hummel, Beissbarth
 Tuesday, May 06 - Exploratory analysis
09.00-10.30 Differential gene expression Ulrich Mansmann
10.30-12.00 Clustering Adrian Alexa
13.30-17.00 Exercises: Differential gene expression
Clustering
Mansmann, Hummel, Alexa
 Wednesday, May 07 - Molecular Diagnosis
09.00-10.30 Molecular Diagnosis Holger Fröhlich
10.30-12.00 Classification with PAM and Random Forest Markus Ruschhaupt
13.30-17.00 Exercises: Molecular Diagnosis Ruschhaupt, Fröhlich, Alexa
 Thursday, May 08 - Pathways
09.00-10.00 Computational Inference of Cellular Networks Achim Tresch
10.15-11.15 Group testing: global tests, holistic approaches Manuela Hummel
11.30-12.30 Scoring Gene Ontology terms Adrian Alexa
13.30-17.00 Exercises: Geneset Enrichment, Global testing, topGO Tresch, Hummel, Alexa

Background Knowledge

Ideally, you are interested in mathematical and statistical problems and are familiar with at least one programming language. This course focusses on the practical side of gene expression data analysis. However, data analysis without understanding the statistical background is in general impossible. We strongly recommend you to refresh your mathematical and programming skills before attending the course.

Please use the links to software and literature to prepare yourself before the course begins.

R and Bioconductor

In the afternoon exercises you will learn how to analyze data using the statistical computing environment R [http://www.r-project.org] and BioConductor [http://www.bioconductor.org], an open source software for bioinformatics. R sources and package sources can be downloaded from The Comprehensive R Archive Network at http://cran.r-project.org.

This is a course in microarray analysis -- not an introduction to R. Please read the Introduction to R before the course begins.

Bring your own data

You are encouraged to bring some of your own data to the course (e.g. genepix files or CEL/CDF). We will use this during the exercises. If you expect to have own data only later in the year, it may in fact be advantageous also to register for one of the later courses.

Participants

Harmjan KuipersLMU Munich -IFIGermany 
Winfried HofmannMedizinische Hochschule Hannover, OE 5120Germany 
Wenjing ZhouUppsala UniversitySweden 
Aleksandra SwierczPoznan University of Technology, Inst. Comp.Sc.Poland 
Anna WidelskaPoznan University of Technology,Inst.Comp.SciencPoland 
Sergey UstinovNational University of Ireland, GalwayIreland 
Inka PraulichMedizinische Hochschule HannoverGermany 
Yinyin YuanWarwick UniversityUK 
Josep Pareja GomezPompeu Fabra UniversitySpain 
sriram kannanindividual trying for PhdIndia 
Diana SantacruzUniversity of SaarlandGermany 
Andreas Mayergene center/LMU81377 MunichCancelled
Guvanch OvezmuradovGenetics Institute University of Cologne Germany 
Michal SwierniakCancer Centre, Institute of OncologyPoland 
Aleksandra PfeiferCancer Centre, Institute of OncologyPoland 
Wouter Van GoolVIB (www.vib.be)9052 GENT, BELGIUM 
Nina PälmkeEpigenetics, University of SaarlandGermany 
Stefano TortiMax Planck Institute for Plant Breeding ResearchGermany 
Stefan FrankeUniversity Hospital, Dept. Ped. OncologyGermany 
Michal JarzabMSC Memorial Cancer Center, GliwicePoland 
Malgorzata Oczko-WojciechowskaMSC Memorial Cancer Center and Institute of OncoPoland 
jeevitesh msIBABINDIA 
Christina PoehlerDKFZGermany 
Salim LardjaneHelmholtz Center Munich, IBBGermany 
Zlatana PasalicHelmholz Zentrum MunichGermany 
Marko PetekNational Institute of BiologySlovenia 
Jana SilhavaFaculty of Information Technology, BUTCzech Republic 
Kim Van DeunGhent UniversityBelgiumCancelled
Vindi JurinovicLMU - IBEGermany 
Zheng YuTU MuenchenGermany 
Romain FenouilCIML centre CNRS INSERMFrance 
Karine Sá FerreiraUniversity of Freiburg - Medical SchoolGermanyCancelled

Thank you for registering! If you will not be able to attend, please contact Tim Beißbarth as soon as possible so that we can notify the people on the waiting list.

How to find us

We meet at the Klinikum Großhadern, Hörsaaltrakt, Floor K O1, Hoersaal I, Marchioninistraße 15, 81377 München.
Großhadern lies in the south-west of Munich (map). A detailed how-to-get-there is found here (in german only). For navigating, you might also find the roadmap and the following directions helpful:
• If you arrive in Munich by plane, take the S-bahn S8 ("Airport line") to Marienplatz.
• If you arrive by train, take any S-bahn or U-bahn to Marienplatz.
• From Marienplatz take U6 to final stop Klinikum Großhadern.
• Leaving the platform, you stand inside a glass pyramid (marked in red in the left figure below).

The red pyramid is the Ubahn-station at which you arrive using U6. The building marked in green is your destination.   This is the inside of the clinical center. The seminarrooms are on the upper right next to the letters "KL".

Accomodation

You will need to make your own accommodation arrangements. We recommend rooms at the Hotel Neumayr, which lies in Großhadern close to the clinical center. If you want to stay closer to the center of Munich, we recommend hotels in Schwabing, which is 15min by U-bahn from Großhadern. Have a look at Hotel Hauser, Hotel Lettl, or Renner Hotel Savoy.

Further information

Please contact Tim Beissbarth: t.beissbarth@dkfz.de.

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