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Courses in Practical DNA Microarray Analysis 2007
2007 May 07-10 in Regensburg
The course is open for registration.
Application: Please send a short note that explains your existing skills and experience with (a) microarrays, (b) statistics, and (c) computer programming and your motivation for taking this course to t.beissbarth@dkfz.de. Additionally, please tell us your NGFN Förderkennziffer (FKZ), if available.
You may use the Registration Form.
If you bring your own laptop, be sure to have installed the following packages:
affy, Biobase, marray, multtest, limma, vsn, arrayMagic, cluster, RColorBrewer, hgu95av2, hgu95av2cdf, estrogen,
ALL, annotate, genefilter, multtest, ROC, isis, pamr, e1071, MCRestimate, GlobalAncova, globaltest, GOstats,
hgu133a, Rgraphviz.
Datasets and additional packages used in the practical sessions can be found in the data directory, links to the tutorial slides and practical exercises in the table below.
Preliminary Course schedule
Monday, May 7 - First Analysis steps |
09.00-09.30 |
Introduction and overview |
Benedikt Brors |
09.30-12.30 |
Quality control, normalization and design |
Tim Beissbarth |
14.00-17.00 |
Exercises: Introduction to R, cDNA data, affy data |
Beissbarth, Brors |
Tuesday, May 8 - Exploratory analysis |
09.00-10.30 |
Differential gene expression |
Anja von Heydebreck |
10.30-12.00 |
Clustering |
Jörg Rahnenfüührer |
12.00-13.00 |
Annotation |
Benedikt Brors |
14.00-17.00 |
Exercises: Differential gene expression Clustering |
Rahnenführer, Heydebreck |
Wednesday, May 8 - Molecular Diagnosis |
09.00-10.30 |
Molecular Diagnosis |
Rainer Spang |
10.30-11.30 |
Classification with PAM and Random Forest |
Markus Ruschhaupt |
11.30-12.30 |
Model Assessment and Selection |
Rainer Spang |
13.30-17.00 |
Exercises: Molecular Diagnosis |
Ruschhaupt, Spang |
Thursday, May 10 - Pathways |
09.00-10.00 |
Computational Inference of Cellular Networks |
Achim Tresch |
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10.15-11.15 |
Group testing: global tests, holistic approaches |
Ulrich Mansmann |
11.30-12.30 |
Scoring Gene Ontology terms |
Adrian Alexa |
13.30-17.00 |
Exercises: Group testing and time to work on your own data |
Mansmann, Hummel, Alexa |
Background Knowledge
Ideally, you are interested in mathematical and statistical problems and are familiar with at least one programming language. This course focusses on the practical side of gene expression data analysis. However, data analysis without understanding the statistical background is in general impossible. We strongly recommend you to refresh your mathematical and programming skills before attending the course.
Please use the links to software and literature to prepare yourself before the course begins.
R and Bioconductor
In the afternoon exercises you will learn how to analyze data using the statistical computing environment R [http://www.r-project.org] and BioConductor [http://www.bioconductor.org], an open source software for bioinformatics.
R sources and package sources can be downloaded from The Comprehensive R Archive Network at http://cran.r-project.org.
This is a course in microarray analysis -- not an introduction to R. Please read the Introduction to R before the course begins.
Bring your own data
You are encouraged to bring some of your own data to the course (e.g. genepix files or CEL/CDF). We will use this during the exercises. If you expect to have own data only later in the year, it may in fact be advantageous also to register for one of the later courses.
Participants
Narendra Kaushik | School of Biosciences, University of Cardiff | United Kingdom | |
Kathrin Juerchott | RZPD - Dt. Ressourcenzentrum fuer Genomforschung | Germany | |
Susanne Haider | Universität Wien | Österreich | |
Anke Thiel | febit biotech gmbH | Germany | |
Caroline Reiff | Rowett Research Institute | UK | |
Laura Giannino | Istituto Sperimentale Italiano Lazzaro Spallanza | Italy | |
Justine Rochon | Zentrum für Klinische Studien (ZKS) Regensburg | Germany | |
Matthias Gondan | University of Regensburg | Germany | Cancelled |
Marijana Radonjic | TNO | The Netherlands | |
Andre Scherag | Institute of Med. Biometry and Epidemiology | Germany | |
Andre Boorsma | University of Maastricht | Netherlands | |
Alexander Reuß | KKS Marburg | Germany | Cancelled |
Christian Baranyi | Uni. Vienna | Austria | |
Adrian Schroeder | University of Tuebingen | Germany | |
Ruben Alcazar | Max Planck Institut für Züchstungforschung | Koeln | |
Marta Bitrian | Max Planck Institute for plant breeding research | Germany | |
Liping Xu | Uni Tuebingen | Germany | |
Nga Lao | Dept. of Genetics, Trinity College | Ireland | |
Dejan Lazarevic | SISSA/ISAS | Italy | |
Helena Krmac | SISSA/ISAS | Italy | |
Nicolas Villacorta | Max Planck Institute for Plant Breeding Research | Germany | |
Benoukraf Touati | CIML INSERM-CNRS | Marseille | |
Ondrej Valina | University of Southern Denmark | Denmark | |
Xue Dong | CUBIC center University Cologne | Germany | |
Andreas Draeger | University of Tuebingen | Germany | Cancelled |
Friederike Trognitz | ARC Seibersdorf research centers | Austria | |
Farzana Abubakar | The Aga Khan University | Pakistan | |
Kalathur Ravi Kiran Reddy | IGBMC | France | Waiting List |
Samuel Arvidsson | Potsdam University | Germany | Waiting List |
Won Kyong Cho | Botanisches Institut in München | Germany | Waiting List |
Dr. G. Madhavi Sastry | W/o Dr. G. Narahari Sastry, AvH fellow, Munich | germany | Waiting List |
Andrea Soltysova | Fac. of sciences UK, dep.of molecular biology | Slovakia | Waiting List |
Susi Barth | blue-drugs GmbH | Germany | Waiting List |
Yasmina Bauer | Actelion | CH | Waiting List |
Kathrin Michel | Max Planck Institut für neurologische Forschung | Germany | Waiting List |
YALÇIN TAHTALI | MAKRO FARMA | istanbul/TURKİYE | Waiting List |
Michael Rehli | University of Regensburg | Germany | Waiting List |
Carol El Chartouni | University of Regensburg | Germany | |
Elmar Schilling | University of Regensburg | Germany | |
Andreas Maurer | Uni Klinikum Regensburg | Germany | |
Thank you for registering!
If you will not be able to attend, please contact Tim Beißbarth as soon as possible so that we can notify the people on the waiting list.
How to find us
We meet Monday, May 07., at 9am at the University Clinic, building A2 seminar room 1, in Regensburg (see map). The clinic can be reached by car from the A3 exit "Regensburg Klinikum" or by public transport from the Regensburg central station using bus line 6 to the final destination "Klinikum" in about 10 minutes. The bus station "Bustreff Albertstrasse" is in viewing distance of the central station around the corner at the "Galgenbergbridge".
Accomodation
You will need to make your own accommodation arrangements. We recommend rooms at the
Hotel Apollo, which is a five minutes walk away from the clinical center.
You could also look for a hotel in the center of Regensburg, which is aproximately 10 minutes by bus. See
map.
Further information
Please contact Tim Beissbarth: t.beissbarth@dkfz.de.
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